Advances in the Preclinical Study of Ischemic Stroke by M. Balestrino

Advances in the Preclinical Study of Ischemic Stroke by M. Balestrino

By M. Balestrino

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Bone marrow samples may be harvested from the iliac crest in humans and in sheep. Therefore, the puncture area on both iliac crests are shaved and disinfected while the anesthetized sheep is placed in a prone position. Samples of approximately 10 mL of bone marrow are taken from both sides by multiple punctures using a heparinized syringe. Higher aspiration volumes from single punctures may result in contamination with peripheral blood. In the next step, the mononuclear cells (MNC) are isolated by density gradient centrifugation, which should be done within one hour after bone marrow harvest.

Sachinidis A. (2007). Epigallocatechin-3-O-gallate inhibits the angiotensin II-induced adhesion molecule expression in human umbilical vein endothelial cell via inhibition of MAPK pathways. Cell Physiol Biochem 20 (6); 859-866. T. M. (2011). Resveratrol attenuates oxidized LDL-evoked Lox-1 signaling and consequently protects against apoptotic insults to cerebrovascular endothelial cells. J Cereb Blood Flow Metab 31 (3): 842-854. W. S. (2010). Calcium- and phosphatidylinositol 3-kinase/Akt-dependent activation of endothelial nitric oxide synthase by apigenin.

Identify positioning of animal by clicking on “Show Image & Detail” (Fig. 2a, 2). 3. g. “actual” orientation  Sphinx Heads First; “scanner” orientation  supine head first) To avoid accidental intrasurgical damage of major cerebral arteries, an overlay with an angiographic data set is recommended. Therefore, use the “Overlays” tab: 1. Implement the data set by clicking “Load Overlay”. 2. Define the desired opacity by choosing the corresponding slider. In the next step, anatomical structures can be defined by segmentation.

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