Aircraft of the Royal New Zealand Air Force by David; Swing, Ross Duxbury

Aircraft of the Royal New Zealand Air Force by David; Swing, Ross Duxbury

By David; Swing, Ross Duxbury

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J , and Gllham, P T. (1981) Tetrahedron Left. 22, 4177-4180 7. , and Wolter, A. (1983) Tetrahedron Lett 24,747-750. 8. Sproat, B. S and Bannwarth, W. (1983) Tetrahedron Lett. 24,5771-5774. , Sheppard, R. , and Rosevear, A. (1981) J Chem. Sot Commun, 1151-l 152 10. Chattopadhyaya, J. B. and Reese, C B (1978) J Chem Sot. Commun. 639-640. 11 Schaller, H , Weimann, B , Lerch, B , and Khorana, H G. (1963) J Am Chem Sot 85,3821-3827 12 Reese, C. S. 182,319-328. 13. Reese, C B , Tltmas, R. C , and Yau, L.

Consequently, the meticulous purification of synthetic oligonucleotides is critical for these specific applications. 6. 1. 2~pool-scale synthesis is evaporated to dryness in vacua. The oligomer is dissolved in water (1 mL) and extracted three times with ethyl ether saturatedwith water (1 mL) to remove deprotection side products, such asbenzamide, isobutyramide, and 4,4’-dimethoxytritanol. Half the volume of the aqueous phase (500 w) is evaporated to dryness under reduced pressure. 2% bromphenol blue: 10x TBE buffer:water [8: 1: 11)(55 pL) is then added to the residue, and after heating in boiling water for 35 min, the solution is quickly chilled on ice.

This methodology has been applied to the synthesis of various monofunctional phosphitylating reagents useful in the synthesis of deoxyribonucleoside phosphoramidites. A selected number of them are listed in Table 1. 3. 1. 85 mol), dropwise, over 1 h under an inert Phosphoramidite 41 Approach Table 1 Chlorophosphoramldlte Derivatives Useful in the Synthew of Deoxyrlbonucleoslde Phosphoramidites Cl / R’O-P \ R’ R” Me- -NMe2 Me- -N(/-Pr), Me- -NnO NCCH2CH2- Me)*- C13CC(Me)2CI&C(Me),- Me i -‘d- 02NeCH2CH2- O,N+CH,CH,- References 1 536 5 12 -Nn Cl&C( R” w” -NMe, 23 -N(/-Pr), 23 ‘\O 23 -Nu CH CH * 22 -NnO 24 -Nn 25 *- -N a w” 26 42 Beaucage atmosphere.

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